Rhodiola crenulata root and rhizome is a popular dietary supplement but may also be considered an adulterant of Rhodiola rosea, a drug used in traditional medicines. This method identifies dried root and rhizome of Rhodiola crenulata (Hook. f. & Thomson). H. Ohba by HPTLC fingerprint, and discriminates root and rhizome of Rhodiola rosea L. as well as Sedum roseum (L.) Scop. and Sedum quadrifidum Pall. The method is included in the USP Herbal Medicine Compendium.